mrpp3 coding sequence (Addgene inc)
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mrpp3 coding sequence
Mrpp3 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrpp3+coding+sequence/pET28-MRPP3-His+(Plasmid+%2367866)/pm41826300-447-43-46
Average 94 stars, based on 1 article reviews
Mrpp3 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrpp3+coding+sequence/pET28-MRPP3-His+(Plasmid+%2367866)/pm41826300-447-43-46
Average 94 stars, based on 1 article reviews
mrpp3 coding sequence - by Bioz Stars,
2026-09
94/100 stars
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Generated:Article Title: Hypusination of the translation factor eIF5A regulates mitochondrial tRNA processing to promote prostate cancer aggressiveness. Article Snippet: To generate cell lines and tumoroids that express IPTG-inducible shDHPS, we infected the cells with lentivirus containing plasmid pLKO-puro-IPTG-3xLacO which holds: Clone TRCN0000330717, ref seq NM_001930 (Sigma-Aldrich) and then the cells were selected AR TI CL E IN P RE SS with puromycin 1μg/ml for 1 week, to inhibit DHPS the cells were induced with IPTG 250μM for 5 days. .. We also generated cell lines and tumoroids expressing shCtl, shMRPP3 and shDHPS by infecting them with lentiviruses containing the plasmids: (pLV[shRNA]-EGFP/NeoU6>Scramble_shRNA), (pLV[shRNA]-EGFP:T2A:NeoU6>hPRORP[shRNA#1](VB900138-9190phn),(pLV[shRNA]-EGFP:T2A:NeoU6>hDHPS[shRNA#1] (VB900141-4950rcu) and we selected resistant clones with Geneticin 400μg/ml (Gibco) for 3 weeks. . To overexpress MRPP3 protein, we inset the Expressing:Article Title: Hypusination of the translation factor eIF5A regulates mitochondrial tRNA processing to promote prostate cancer aggressiveness. Article Snippet: To generate cell lines and tumoroids that express IPTG-inducible shDHPS, we infected the cells with lentivirus containing plasmid pLKO-puro-IPTG-3xLacO which holds: Clone TRCN0000330717, ref seq NM_001930 (Sigma-Aldrich) and then the cells were selected AR TI CL E IN P RE SS with puromycin 1μg/ml for 1 week, to inhibit DHPS the cells were induced with IPTG 250μM for 5 days. .. We also generated cell lines and tumoroids expressing shCtl, shMRPP3 and shDHPS by infecting them with lentiviruses containing the plasmids: (pLV[shRNA]-EGFP/NeoU6>Scramble_shRNA), (pLV[shRNA]-EGFP:T2A:NeoU6>hPRORP[shRNA#1](VB900138-9190phn),(pLV[shRNA]-EGFP:T2A:NeoU6>hDHPS[shRNA#1] (VB900141-4950rcu) and we selected resistant clones with Geneticin 400μg/ml (Gibco) for 3 weeks. . To overexpress MRPP3 protein, we inset the Clone Assay:Article Title: Hypusination of the translation factor eIF5A regulates mitochondrial tRNA processing to promote prostate cancer aggressiveness. Article Snippet: To generate cell lines and tumoroids that express IPTG-inducible shDHPS, we infected the cells with lentivirus containing plasmid pLKO-puro-IPTG-3xLacO which holds: Clone TRCN0000330717, ref seq NM_001930 (Sigma-Aldrich) and then the cells were selected AR TI CL E IN P RE SS with puromycin 1μg/ml for 1 week, to inhibit DHPS the cells were induced with IPTG 250μM for 5 days. .. We also generated cell lines and tumoroids expressing shCtl, shMRPP3 and shDHPS by infecting them with lentiviruses containing the plasmids: (pLV[shRNA]-EGFP/NeoU6>Scramble_shRNA), (pLV[shRNA]-EGFP:T2A:NeoU6>hPRORP[shRNA#1](VB900138-9190phn),(pLV[shRNA]-EGFP:T2A:NeoU6>hDHPS[shRNA#1] (VB900141-4950rcu) and we selected resistant clones with Geneticin 400μg/ml (Gibco) for 3 weeks. . To overexpress MRPP3 protein, we inset the Sequencing:Article Title: Hypusination of the translation factor eIF5A regulates mitochondrial tRNA processing to promote prostate cancer aggressiveness. Article Snippet: To generate cell lines and tumoroids that express IPTG-inducible shDHPS, we infected the cells with lentivirus containing plasmid pLKO-puro-IPTG-3xLacO which holds: Clone TRCN0000330717, ref seq NM_001930 (Sigma-Aldrich) and then the cells were selected AR TI CL E IN P RE SS with puromycin 1μg/ml for 1 week, to inhibit DHPS the cells were induced with IPTG 250μM for 5 days. .. We also generated cell lines and tumoroids expressing shCtl, shMRPP3 and shDHPS by infecting them with lentiviruses containing the plasmids: (pLV[shRNA]-EGFP/NeoU6>Scramble_shRNA), (pLV[shRNA]-EGFP:T2A:NeoU6>hPRORP[shRNA#1](VB900138-9190phn),(pLV[shRNA]-EGFP:T2A:NeoU6>hDHPS[shRNA#1] (VB900141-4950rcu) and we selected resistant clones with Geneticin 400μg/ml (Gibco) for 3 weeks. . To overexpress MRPP3 protein, we inset the Plasmid Preparation:Article Title: Hypusination of the translation factor eIF5A regulates mitochondrial tRNA processing to promote prostate cancer aggressiveness. Article Snippet: To generate cell lines and tumoroids that express IPTG-inducible shDHPS, we infected the cells with lentivirus containing plasmid pLKO-puro-IPTG-3xLacO which holds: Clone TRCN0000330717, ref seq NM_001930 (Sigma-Aldrich) and then the cells were selected AR TI CL E IN P RE SS with puromycin 1μg/ml for 1 week, to inhibit DHPS the cells were induced with IPTG 250μM for 5 days. .. We also generated cell lines and tumoroids expressing shCtl, shMRPP3 and shDHPS by infecting them with lentiviruses containing the plasmids: (pLV[shRNA]-EGFP/NeoU6>Scramble_shRNA), (pLV[shRNA]-EGFP:T2A:NeoU6>hPRORP[shRNA#1](VB900138-9190phn),(pLV[shRNA]-EGFP:T2A:NeoU6>hDHPS[shRNA#1] (VB900141-4950rcu) and we selected resistant clones with Geneticin 400μg/ml (Gibco) for 3 weeks. . To overexpress MRPP3 protein, we inset the |